Multiple-beam interference: structural colours, iridescence ...
|Diffraction on a single slit. Top: monochromatic light, bottom: white light. In the following we assume that the slits are very narrow and only look at what happens within the central bright region. The dark bands in the left picture would be far outside of the details shown in the following images.|
Diffraction by a double slit|
A plane wave coming in from the left falls on an absorbing screen with two narrow slits. The outgoing waves on the right show alternating constructive and destructive interference. Double-click on the picture will switch on the animation, a single click switches it off again.
|Diffraction pattern of a double slit. Top: monochromatic light. This pattern corresponds to the animated picture above. |
White light produces a coloured pattern (which in most experimental situations is rather dim and therefore the colours are not clearly seen).
|A simple home experiment: Diffraction by a double hole. Two tiny holes were pierced with a sewing needle into an aluminium wrapping foil. Looking through these holes at a "point-like" light source, one can see a diffraction image similar to that shown here. For this image, the pierced foil has been fastened in front of the lens of a digital camera, the light source was the reflection of a halogen lamp in a small silvery glass ball (as used for christmas-tree decoration). The diameter of the holes was 0.07 mm, the centre-to-centre distance 0.3 mm.|
Three slits. Adding a third slit at the same distance, the intensity maxima are preserved. Between them there are now two dark bands and a faint secondary maximum.|
The pattern produced by white light shows more intense colours.
Seven equidistant slits: the bright "lines" are now separated by six dark bands and five faint secondary maxima.|
The colours of the "first order" diffraction maximum on both sides of the central white beam approach saturated spectral colours. The short-wave (blue-violet) end of the third-order diffraction already overlaps the long-wave (red) end of the second order.
|Everybody knows the vivid colours seen on compact discs. There is a mirror image showing no colour-splitting, but depending on the viewing angle, diffraction images of first or higher order can be seen. In case of small light sources, in first order almost pure spectral colours appear.|
Under favourable conditions (dark background, but against the light) also spider webs may shine in vivid colours. For that, there are different possible reasons. In the picture shown, the main effect is due to the regular array of the glittering tiny sticky droplets on the capture threads. The web is shown approximately in natural size, intentionally slightly out of focus. |
The pages "spider webs" present more images and give more detailed explanations.
|If instead of simple stripes a more involved pattern is produced, colourful images may result, as in the case of holograms. Best known examples probably are those seen on bank-notes.|
|Birds' feathers consist of a hollow shaft with branches fused to it on opposite sides. The branches (barbs) carry barbules which in the feathers' vanes are so small, dense, and regularly ordered that they form a diffraction grating. The picture on the left shows the feather of a pigeon, illuminated by a white LED in the background. Below at the left an enlarged detail where the focussing was on the feather, at the right the same with focus on the light source in the background.|
|Reflectivity and colour of a stack of 16 double layers as a function of the viewing angle. Refractive indices are 1.45 and 1.6, optical thickness of a double layer is 300 nm.|
Details of the computation of the above pictures for stacked layers are given in the articles collected in the section "Computing Colours"; there are also sample codes for the images.
|Scanning electron micrograph of a transverse fracture of nacre. Photo: Fabian Heinemann, source: http://de.wikipedia.org. Click on the image for enlargement.|
consists of thin aragonite tiles held together by layers of an organic polymer (conchiolin, chitin or other).
At each boundary between the crystals and the polymer, part of the incident light is reflected. Depending on their wavelength, the reflected waves may interfere constructively or destructively.
The layers between the tiles are thin. From a scanning electron micrograph of a transverse section of the shell of Haliotis glabra in a paper by T.L. Tan, D. Wong, and P. Lee, Optics Express 12 (2004), No. 20 p. 4847, (link) one can see that it is about one third of the thickness of the tiles or less.
The image above shows computed colours for a stack of 32 layers. Normal incidence is assumed for simplicity, the refractive indices are taken to be 1.685 (aragonite) and 1.57 (chitin), and the ratio of optical thicknesses 85:15.
As the colours depend sensitively on the parameters, exact agreement can not be expected. But a general property can be seen: vivid colours arise for thin layers; for a periodicity interval above 2 μm only pale greens and pinks are seen, which are the most common nacreous colours.
|Reflectivity and colour of a stack of 1024 double layers, parameters as in the previous picture, optical thickness of a double layer 1480 nm.|
|Striated muscles under the microscope (stained). The length of the bar in the lower right corner is 30 μm. |
© 2012 The University of Kansas Medical Center; authors: Milton Wolf & Marc Scarbrough.Source: The JayDoc HistoWeb
|Transmission elektron micrograph (TEM) of human striated muscle. |
Image width: 10 μm. Photo: Louisa Howard,
Moonstone, another feldspar mineral, consists of orthoclase or microcline KAlSi3O8 with a small admixture of albite NaAlSi3O8. It shows an attractive pale bluish lustre called adularescence, from adularia, which is another name for moonstone. The sheen is caused by the layered structure of alternating potassium rich and sodium rich lamellae.
A strictly regular stack of alternating layers with different refractivity would produce strong iridescence, i.e. colours changing with the viewing angle, as demonstrated above in the case of labradorite. If, however, the orthoclase layers (which are much thicker than the albite lamellae, according to the composition of the stone) deviate randomly from their average thickness, the interference between the waves reflected from the different thin albite sheets is averaged out and the reflected intensities add up.
Transparent films which are much thinner than the wavelengths of light reflect short waves more than long ones (the reflectivity is then inversely proportional to the square of the wavelength). Therefore, with white illumination, the reflected light is bluish. With increasing thickness, the reflection becomes white, then yellowish.
|A sample of a cholesteric liquid crystal enclosed between acrylic glass and a black plastic backing. Such gadgets were popular in the 1970s as decorative objects or toys ("Space Fidgets"). The colour depends on the viewing angle. Touching the disc on the back side produces changing iridescence when the fluid elements are deformed.|
|As a surprise, in both polarizations colours are seen. Though the covering plate may be somewhat birefringent which would change the polarisation of the reflected light, so that it is no longer 100 % circular, the explanation is that
there is not a uniform helicity in the alignment of the fluid, instead, both helicities occur with the right handed one prevailing. The large dark region in the left picture, where the liquid layer is very thin, represents near to ideal conditions and there, left handed light is almost absent. |
The image to the right has been taken through a microscope. The width is 1 mm. The liquid is not homogeneously layered, but instead the orientation of the planes is constant only over small parts of the volume, which leads to a grainy, glinting appearance.
|First a preliminary remark. Iridescence may be distinguished from the gloss of the surface, if the iridescent structures in the volume are not exactly parallel to the surface. Where a dark object is mirrored at the surface, the light coming from deeper layers is seen (right image). The following photographs have been taken avoiding the bright reflections on the surface.|
|Reflectivity and colour of a cholesteric liquid crystal. Model computation with parameters nparallel = 1.60, northogonal = 1.45, absorption coefficient κ = 0.002, pitch of the helical ordering H = 393.44 nm (corresponding to the optical periodicity interval of 300 nm), total height D = 4 μm.|
Butterfly wings are covered with microscopic, partially overlapping scales. White, yellow, orange, red, brown, grey, or black colour of the scales can originate from their pigmentation, green pigments, however, are rare, and blue ones are very rare. In most cases, green and blue on butterflies' wings are structural colours; the other colours may occasionally be structural colours as well. |
The image to the right shows pigmented and unpigmented scales from the wing of the Small Tortoiseshell Aglais urticae. The image width is 0.8 mm.
Cross section of an iridescent scale of Urania sp. (Source: pages of the School of Physics at The University of Exeter)
|Urania riphaeus from Madagaskar. (Source: FAAXAAL, public domain photos for the internet. Author: Kriss de Niort)|
|Morpho rhetenor cacica from South America. Specimen of the Insects' Museum in Wunstorf/Steinhude, wingspan ca. 15 cm.||Part of a cross section of an iridescent scale of Morpho aega. Elektron microscope picture: W. Lippert. (From: Karl Gentil, Interferenzfarben ... , Zeiss Informationen 49, p. 80–81, about 1966)|
Each of the strips consists of a regular arrangement of thin transparent chitinous layers. Altogether these form an array of reflecting surfaces, and the considerations are similar to those sketched in the case of opal. For a more thorough discussion, see this OPOD of the atmospheric optics site.
It is remarkable that the structural colours of many butterflies are hardly or not at all pavonine. How iridescence is suppressed has been investigated in a few species, among them the Blue Celastrina argiolus and the Green Hairstreak Callophrys rubi (Poladian et al. 2008).
Reflectivity and colour of four chitin layers separated by three air-filled gaps. Optical thickness of each layer/gap is D = 127.5 nm. In view of the large changes of the colour it is marvelous how evolution has succeeded to produce structural colour without iridescence.|
The blues, like the holly blue, Celastrina argiolus, use stacks of layers with air gaps in between, however, the layers are not continuous, but rather like an irregular network. Moreover, the upper surface is not smooth, but structured. This reduces the dependence of the colour on the viewing angle.
Right: Holly blue, Celastrina argiolus
|The scales of the green hairstreak, Callophrys rubi, contain regular cubic lattice structures (gyroid structures), see Michielsen et al. 2007, figures 5–7. On each scale, there are many small randomly oriented domains of few μm diameter only. By this, iridescence is averaged out and the scales look uniformly coloured (Poladian et al. 2008).|
See also the TEM images from the paper of Prum et al. (2006) Fig. 4, Fig. 5 .
Different mechanisms can be found in beetles to produce lustrous colours.
Tropical jewel beetles of the species Chrysochroa raja have been investigated by Noyes et al. (2007) who found multilayer structures with alternating index of refraction.
The leaf-beetle Plateumaris sericea (L.) occurs in various hues. There is a simple sequence of layers at the surface: three layers with high refractive index, between them thicker layers with lower index. The layers between 50 and 120 nm thick in one special case (Hariyama et al. 2002).
In the following two examples, the same method of colour generation may be taken for granted, only the number of layers is not yet known.
Dead-nettle leaf beetle
Cylindrical leaf beetle
|Many beetles (of the family Scarabaeidae) create their metallic colour in a different way. In layers the chitin molecules are aligned parallel to each other and to the surface. From one layer to the next the orientation angle changes by a small amount (like the steps in a corcscrew staircase), leading to a helical structure (similar to the ordering of molecules in a cholesteric liquid crystal, see above.).||
Rose chafer Cetonia aurata L.
Two corresponding images of a flower chafer, species Protaetia cuprea (= Protaetia metallica).
It is remarkable that the beetle looks metallic, iridescent colour changes are hardly noticeable. This is probably achieved by the microstructure of the surface, microscopic wrinkles, dimples or pimples. It would be interesting to see SEM images.
In beetles of the genus Plusiotis/Chrysina from South America, the helical molecular order in the cuticle has been discovered first, and species from this genus are still preferentially investigated. Jewell et al. (2007) found microscopic dimples in the elytra of Plusiotis boucardi, Sharma et al. (2009) the same in Chrysina gloriosa (synonym Plusiotis gloriosa).
For weevils, three-dimensional, opal-like structures have been identified as the mechanism producing sheeny colours. These regular structures are tiny and more or less randomly oriented in the scales on the surface of the insect. It is the same method of reducing iridescence which has been described for the green hairstreak above.
|The weevil Phyllobius pomaceus Gyllenhal (Green Nettle Weevil, length 9 mm without antennae) is covered with greenish shimmering scales.||Elytron of Phyllobius pomaceus under the microscope. The width of the image is 0.5 mm (click for enlargement).|
|A specimen of Polydrusus sp. (length 6 mm, found dead) under the microscope.||The elytron at higher resolution. The image width is 0.5 mm.|
Some exotic weevils use this mechanism for fancy colours, see e.g. the picture shown here together with an abstract of an interesting article in "Science".
An overview of all known sources of structural colour of beetles is given in the review article by Ainsley E. Seago et al., "Gold bugs and beyond: a review of iridescence and structural colour mechanisms in beetles (Coleoptera)", abstract with link to free download of the full text.
Peacock's feathers are the best known example of birds' iridescence.
In the cortex of the barbules, under a thin outer skin of keratin, several layers of melanin rods are arranged in a regular pattern. The rods are parallel to the surface and are held together by keratin. The cross section shows that they form a square lattice, with hollow spaces in the centres of the squares (Durrer 1962*, Zi et al. 2003).
Ducks and Pheasants
The black feathers on the head of the mallard drake (Anas platyrhynchos) are glistening green, or glistening blue if the angle between illumination and viewing is increased.
Glossy feathers of different species of ducks and pheasants have been investigared. Here also layers of melanin rods in the keratin matrix of the cortex have been found, but the arrangement of the rods is different from that in peacock feathers. The cross section shows a regular hexagonal, honeycomb-like lattice. (Rutschke 1966)*.
Hummingbirds produce their brillant colours with multilayer microstructures, stacked films made of melanin platelets containing air bubbles, effectively constituting alternating melanin and air layers.
Hummingbird Colibri thalassinus
Photo: Mdf, source: Wikimedia Commons
Hummingbird Calypte costae
Photo: Jon Sullivan, source: PDPhoto.org
Similar structures are employed by the Trogonidae. Layers of hollow melanin platelets, but also of hollow tubes of melanin. (A survey of the research on structural colours is given by Kinoshita 2008*.)
The green colour of reptiles arises from a combination of structural and pigmentary colour. On top there is a layer of xanthophores, i.e. cells containing yellow pigment which absorbs the blue light. Beneath is a layer of guanophores or iridophores, cells which contain nanocrystals of guanine suspended in the cytoplasm. If arranged irregularly, the guanine crystals scatter "Tyndall-like", the longer waves much less than the shorter ones. "Blue" is already absorbed, so what remains to be reflected is green. A bottom layer of melanophores provides a dark background.
If the guanine nanocrystals are arranged regularly, in addition to blue also other structural colours are possible, as in layered structures or opal.
A chameleon of Madagascar
(Photo: Mila Zinkova)
|Chameleons are famous for their ability to change colour. In this, different mechanisms may be involved: the ability of the chromatophores to spread the pigment particles all over the cell or to concentrate them into a small lump, or, as published in 2015, tuning of the iridophores.|
Teyssier, J. et al. (2015) investigated the chameleon Furcifer pardalis from Madagascar. They found iridophores where the guanine nanocrystals form a regular face-centered cubic lattice. This is similar to the close-packing of spheres in opal, but the crystals do not touch. The orientation of the lattices is random, so that iridescence is averaged out. The colour is changed by changing the osmotic pressure, inducing swelling or shrinking of the iridophores by which the distance between neighbouring crystals is increased or reduced.
One-, two- or three-dimensional periodic arrays of refracting or absorbing elements in a matrix are called photonic crystals. (These arrays show band structures for light waves = photons which are similar to the energy bands of electrons = matter waves in crystals, hence the name.)
One-dimensional stacks of dielectric layers on glass are used as dielectric mirrors and colour filters. Two examples are shown in the images below.
Two- and three-dimensional photonic crystals are fields of research in nanotechnology and could perhaps soon find practical applications, while evolutionary nano-engineering has already produced wonderful examples in birds' feathers and butterflies' wings.
Diffraction vs. multilayer interferenceThree ways of generating colour have been discussed here. The first is diffraction on grid-like surface structures, the second is interference of specular reflections on multilayer or three-dimensional lattice structures, without noticeable diffraction, and the third is reflection due to helically periodic variation of the refractive index. As in all cases the iridescent structures are sub-microscopic, one may wonder how to discriminate between them. (Of course, also in the first case the colours are due to interference of the diffracted waves.)
To the right there is a gelatine-film replica of a diffraction grating with 13400 grooves per inch and the nacre shell of Haliotis asinina, in the light of an incandescent lamp.
|The change in angle from the first one to the last is so small that the perspectivic distortion of the figure is hardly noticeable. Thus, even if we would not know that it is a hologram, we could infer that diffraction is the cause of the colours.|
In addition, in this case there is (almost) always the zero order reflection without colours, as shown on the right-hand side. Colours are only seen if this specular reflection is avoided. Under diffuse illumination like the overcast sky no colours or only very faint ones can be seen.
|Moreover, to see diffraction effects, one needs a light source with small angular extension. If there is only diffuse light as from the overcast sky, no diffraction effects are visible on the grating at any angle, while the nacre is shimmering in delicate pink and green.|
|Colours due to thin films, multilayer structures in particular, persist under diffuse light. The image to the right shows a polished specimen of labradorite from Madagascar, with a CD in the background which does not show any colour.|
|–||Heinz Durrer: Schillerfarben beim Pfau (Pavo cristatus L.): eine elektronenmikroskopische Untersuchung. Arbeiten aus der zoologischen Anstalt Basel, 21 Seiten, 1962|
|–||Shuichi Kinoshita: Structural Colors in the Realm of Nature. World Scientific 2008, ISBN-13 978-981-270-783-3|
|–||E. Rutschke (1966): Die submikroskopische Struktur der Federn von schillernden Entenvögeln. Z. Zellforsch. 73, 432–443|
|–||J. Teyssier, S.V. Saenko, D. van der Marel & M.C. Milinkovitch: Photonic crystals cause active colour change in chameleons. Nat. Commun. 6:6368 doi:10.1038/ncomms7368 (2015)|